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ROCANAL
Root Canal Therapy

by Dr. Edgar J. Corneo-Lobenstein

A Biotechnical System Part I
A Biotechnical System Part II
4 Clinical Cases
User Guide
Bibliography

A Biotechnical System Part II - Page 3

TABLE 1: In Vitro Test on Microbicidal Efficiency of PVP-Iodine

Micrococcus pyrogenes var. aureus (FDA 209 and an antibiotic-resistant stain) Salmonella typhosa (2 stains) Streptococcus hemolyticus (2 strains) Pseudomonas aeruginosa (2 strains)	PVP-Iodine (1% available iodine) in dilutions up to 1 : 10 without and with 10% whole blood or 5% serum killed on contact within 15 sec all bacteria in 4 mm loop.
Micrococcus pyrogenes var aureus	PVP-Iodine (1% available iodine) sterilized the culture; tincture of iodine beaker contained viable cout of 1040 bacteria per ml.
Streptococcus pyrognes Pseudomonas aeruginosa Escherichia coli Micrococcus pyrogenes var. aureus Candida albicans Trichophyton mentographytes	PVP-Iodine (1% availabel iodine) killed organisms faster than other preparations containig nitrofuran, zinc undecylenate, thiomerosal, cetylpyridinium chloride, quaternary ammonium, chlorothymol, and merbromin.
Staphylococcus aureus Streptococcus pyogenes Diplococcus pneumoniae Proteus mirabilis Pseudomonas aeruginosa Bacillus subtilits	PVP-Iodine (1% available iodine) in dilution of 1 : 2 killed on contact within 90 sec approximately 95% of each test organism; in dilution 1 : 10, 60 - 70%
Staphylococcus aureus (FDA 209 and 3 antibiotic-resistant strains) Staphylococcus albus Streptococcus pyrogenes (hemolytic and viridans) Diplococcus pneumoniae (types I, II, III, IV, V, VII, VIII and XIV) Neisseria gonorrhoeae Haemophilus vaginalis Candida (Monilia) albicans Trichomonas vaginalis	PVP-Iodine was the only commercial preparation tested that in "recommended use dilution" (1% available iodine diluted 1 : 4) killed all the test organisms within 30 sec. It was effective within 90 sec when diluted 1:40.

TABLE 2: Microbiostatic and Microbicidal Titers of Hyamine 1622

Organism

Dilution of Active Ingredient

Bactericidal	Bacteriostatic

Streptococcus pyogenes C-203
Streptococcus viridans
Escherichia coli

1: 50,000
1: 200,000
1: 16,000

1 : 50,000
1 : 400,000
1 : 16,000

Salmonella gallinarum
Salmonella choleraesuis
Salmonella typhimurium
Salmonella schottmuelleri

1 : 32,000
1 : 32,000
1 : 16,000
1 : 8,000

1 : 32,000
1 : 32,000
1 : 16,000
1 : 32,000

Pseudomonas aeruginosa PRD-10
Lactobacillus casei
Shigella sonnei
Klebsiella pneumoniae

1 : 8,000
1 : 50,000
1 : 16,000
1 : 16,000

1 : 8,000
1 : 100,000
1 : 32,000
1 : 16,000

Saccharomyces cerevisiae
Pityrosporum ovale
Trichophyton interdigitale
Monilia albicans

1 : 100,000
1 : 800,000
1 : 20,000
1 : 400,000

1 : 100,000
1 : 800,000
1 : 40,000
1 : 800,000

Aspergillus niger
Aspergillus oryzae
Penicillium notatum
Penicillium luteum

1 : 400
1 : 800,000
1 : 800,000
1 : 400,000

1 : 800
1 : 1,600,000
1 : 800,000
1 : 800,000

TABLE 3: Antifungal and Antibacterial Efficacies of OPP

Test Organism	% for Inhibition
Fungi Rhizopus nigricans Rhizoctonia solani Chaetomium globosum (ATCC 6205) Hormiscus gelatinosum Aspergillus niger Polyporus tulipiferae (F.P.L. No. 517, ATCC 11245) Aspergillus flavus (ATCC 9643) Lenzites trabea (ATCC 11539) Ceratostomella pilifera Trichophyton interdigitale Trichophyton rosaceum	0.015 - 0.02 0.001 - 0.002 0.0025 - 0.005 0.005 - 0.01 0.025 - 0.05 0.005 - 0.01 0.005 - 0.01 0.0025 - 0.005 0.005 - 0.01 0.002 - 0.0035 0.0035 - 0.005
Bacteria Staphylococcus aureus (ATCC 6538) Bacillus subtilis (ATCC 8473) Enterobacter aerogenes (ATCC 13048) Klebsiella pneumonaiae (ATCC 8308) Pseudomonas aeruginosa (ATCC 10145) Pseudomonas aeruginosa (ATCC 15442) Proteus vulgaris (ATCC 881) Escherichia coli (ATCC 11229) Salmonella choleraesuis (ATCC 10708)	0.01 - 0.015 0.01 - 0.015 0.01 - 0.015 0.01 - 0.015 0.02 - 0.025 0.045 - 0.05 0.005 - 0.01 0.01 - 0.015 0.01 - 0.015
Phenol Coefficients (A.O.A.C. Method, Tested at pH 8 to 11, 20?C) Salmonella typhi (ATCC 6539) Staphylococcus aureus (ATCC 6538) Pseudomonas aeruginosa (ATCC 15442)	24 20 14

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